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In order to validate the effect of GLY on the antioxidant capacity of a TNF-/D-GalN-induced L02 cell injury model, ROS and MDA levels were detected

PubMed Central, doi:10.1162/jocn_a_00942

Plasmid transfection and luciferase reporter assay The luciferase-based reporter plasmids pNFKB-Luc and pTA-Luc (Takara Bio, San Jose, USA) were applied with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturers instructions

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The reduced form is the active, ready-to-work version